Optical Sciences

Biomolecules and nanostructures

The Optical Sciences group studies the interaction of light and matter. Our current focus is on detection and sensing/imaging with an emphasis on the development of integrated photonics. We are part of Twente University's Department of Science and Technology and member of the MESA+ institute.

 

ANALYSIS OF IMMUNOLABELED CELLS BY ATOMIC-FORCE MICROSCOPY, OPTICAL MICROSCOPY, AND FLOW-CYTOMETRY


NEAGU C, VANDERWERF KO, PUTMAN CAJ, KRAAN YM, DEGROOTH BG, VANHULST NF, GREVE J
JOURNAL OF STRUCTURAL BIOLOGY
vol 112 issue 1: p32-p40 JAN-FEB 1994

In this study we investigated the applicability of the (silver- enhanced) immunogold labeling method for atomic force microscopy. Human lymphocytes were labeled with anti-CD3 conjugated to fluorescein isothiocyanate and a secondary antibody (goat anti-mouse) linked with 1- or 30-nm colloidal gold particles. Silver enhancement was applied o­n these labeled cells to increase the size of the labels. In a setup combining an inverted optical microscope and a stand-alone atomic force microscope, a direct correlation was made between the force and the fluorescent images. Additionally, we performed how cytometric analysis. From the results we conclude that immunogold labeling using small labels (1 nm) in combination with silver enhancement (30 min) proves to be a reliable method for high-resolution cell surface antigen detection in atomic force microscopy. (C) 1994 Academic Press, Inc.
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