Biomolecules and nanostructures
The Optical Sciences group studies the interaction of light and matter at the nanoscale.
We do this by exploring ways to shape light and its environment. It's what we call
active and passive control. Our current focus is on the interaction of light with
biomolecules and nanostructures. We are part of Twente
University's Department of Science and Technology and member of the
Vibrational phase contrast CARS microscopy for quantitative analysis(full pdf)
M. Jurna, E.T. Garbacik, J.P. Korterik, C. Otto, J.L. Herek, H.L. Offerhaus
Vol. 7569 p. 75690F-1 February 26, 2010
In biological samples the resonant CARS signal of less abundant constituents can be overwhelmed by the non-resonant background, preventing detection of those molecules. We demonstrate a method to obtain the phase of the oscillators in the focal volume that allows discrimination of those hidden molecules. The phase is measured with respect to the local excitation fields using a cascaded phase-preserving chain. It is measured point-by-point and takes into account refractive index changes in the sample, phase curvature over the field-of-view andinterferometric instabilities. The detection of the phase of the vibrational motion can be regarded as a vibrational extension of the linear (refractive index) phase contrast microscopy introduced by Zernike around 1933.