Optical Sciences

Biomolecules and nanostructures

The Optical Sciences group studies the interaction of light and matter. Our current focus is on detection and sensing/imaging with an emphasis on the development of integrated photonics. We are part of Twente University's Department of Science and Technology and member of the MESA+ institute.

 

POLARIZATION CONTRAST IN FLUORESCENCE SCANNING NEAR-FIELD OPTICAL REFLECTION MICROSCOPY

(full pdf)

JALOCHA A, VANHULST NF
JOURNAL OF THE OPTICAL SOCIETY OF AMERICA B-OPTICAL PHYSICS
vol 12 issue 9: p1577-p1580 SEP 1995

Polarization contrast is presented in fluorescence images of a Langmuir-Blodgett monolayer obtained with a scanning near-field optical microscope operated in reflection. A tapered optical fiber is used both to excite and to collect the fluorescence. The lateral resolution in the reflection fluorescence image is estimated at 200 nm. Shear-force detection controls the tip-to- surface distance and simultaneously gives a topographic map. Direct correlation between topography and the fluorescent domain is thus obtained. We show that the fluorescence is polarized along the molecular orientation in the Langmuir- Blodgett monolayer. Thus we demonstrate the study of the polarization direction in the reflection mode.
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