Biomolecules and nanostructures
The Optical Sciences group studies the interaction of light and matter at the nanoscale.
We do this by exploring ways to shape light and its environment. It's what we call
active and passive control. Our current focus is on the interaction of light with
biomolecules and nanostructures. We are part of Twente
University's Department of Science and Technology and member of the
Fluorescence in situ hybridization on human metaphase chromosomes detected by near-field scanning optical microscopy
Moers MHP, Kalle WHJ, Ruiter AGT, Wiegant JCAG, Raap AK, Greve J, DeGrooth BG, VanHulst NF
JOURNAL OF MICROSCOPY-OXFORD
vol 182: p40-p45 part 1 APR 1996
Fluorescence in situ hybridization on human metaphase chromosomes is detected by near-field scanning optical microscopy. This combination of cytochemical and scanning probe techniques enables the localization and identification of several fluorescently labelled genomic DNA fragments on a single chromosome with an unprecedented resolution. Three nucleic acid probes are used: pUC1.77, p1-79 and the plasmid probe alpha-spectrin. The hybridization signals are very well resolved in the near-field fluorescence images, while the exact location of the probes can be correlated accurately with the chromosome topography as afforded by the shear force image.