Biomolecules and nanostructures
The Optical Sciences group studies the interaction of light and matter. Our current focus is on detection and sensing/imaging with an emphasis on the development of integrated photonics. We are part of Twente University's Department of Science and Technology and member of the MESA+ institute.
Fluorescence in situ hybridization on human metaphase chromosomes detected by near-field scanning optical microscopy
Moers MHP, Kalle WHJ, Ruiter AGT, Wiegant JCAG, Raap AK, Greve J, DeGrooth BG, VanHulst NF
JOURNAL OF MICROSCOPY-OXFORD
vol 182: p40-p45 part 1 APR 1996
Fluorescence in situ hybridization on human metaphase chromosomes is detected by near-field scanning optical microscopy. This combination of cytochemical and scanning probe techniques enables the localization and identification of several fluorescently labelled genomic DNA fragments on a single chromosome with an unprecedented resolution. Three nucleic acid probes are used: pUC1.77, p1-79 and the plasmid probe alpha-spectrin. The hybridization signals are very well resolved in the near-field fluorescence images, while the exact location of the probes can be correlated accurately with the chromosome topography as afforded by the shear force image.