Optical Sciences

Biomolecules and nanostructures

The Optical Sciences group studies the interaction of light and matter. Our current focus is on detection and sensing/imaging with an emphasis on the development of integrated photonics. We are part of Twente University's Department of Science and Technology and member of the MESA+ institute.

 

Near-field scanning optical microscopy of single fluorescent dendritic molecules


Veerman JA, Levi SA, van Veggel FCJM, Reinhoudt DN, van Hulst NF
JOURNAL OF PHYSICAL CHEMISTRY A
vol 103 issue 51: p11264-p11270 DEC 23 1999

Individual dendritic molecules adsorbed o­n glass containing a single fluorescent rhodamine B core have been observed with near-field scanning optical microscopy (NSOM); height and fluorescence images were obtained simultaneously. The dendritic assemblies can be discriminated from free fluorescent cores o­n the basis of accurate simultaneous localization of both the fluorescent core and the surrounding dendritic shell. There are no significant differences between the photophysical properties of the free and dendritic fluorophores. The full three- dimensional orientation of each individual fluorescent core can be resolved and millisecond time resolution accuracy has been achieved. Most dendritic structures exhibited rotational motion of the fluorescent core o­n a millisecond-second time scale, revealing intramolecular conformational dynamics.
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