Optical Sciences

Biomolecules and nanostructures

The Optical Sciences group studies the interaction of light and matter. Our current focus is on detection and sensing/imaging with an emphasis on the development of integrated photonics. We are part of Twente University's Department of Science and Technology and member of the MESA+ institute.

 

Dr. María García-Parajó - Former Member
 



In October 2005, María García-Parajó moved to the University of Barcelona, Laboratori de Recerca en Nanobioenginyeria (CREBEC).



Contact Information

Email: mgarciapcb.ub.es
Phone:+34 93 403 96 15


Former Projects


Articles by María García-Parajó

Refer to Laboratori de Recerca en Nanobioenginyeria (CREBEC) for recent publications of María García-Parajó

Optical antennas focus in on biology

(abstract) (full pdf)
Maria F. Garcia-Parajo
Nature Photonics
vol. 2 issue 4 p. 201-203 april 2008
doi:10.1038/nphoton.2008.37

Memory in single emitter fluorescence blinking reveals the dynamic character of nanoscale charge tunneling

(abstract)
Hoogenboom JP, Hernando J, Garcia-Parajo MF, van Hulst NF
Journal of Physical Chemistry C
vol. 112 issue 9 p3417-3422 march 6, 2008
doi:10.1021/jp710100b

Nanometer-scale organization of the alpha subunits of the receptors for IL2 and IL15 in human T lymphoma cells

(abstract) (full pdf)
Bärbel I. de Bakker, Andrea Bodnár, Erik M. H. P. van Dijk, György Vámosi, Sándor Damjanovich, Thomas A. Waldmann, Niek F. van Hulst, Attila Jenei, and María F. Garcia-Parajo
Journal of Cell Science
vol 121 issue 5 p627-633 feb 20 2008
doi:10.1242/jcs.019513

Ultrafast single-molecule photonics: Excited state dynamics in coherently coupled complexes

(abstract) (full pdf)
Jordi Hernando, Jacob Hoogenboom, Erik van Dijk, Maria Garcia-Parajo, Niek F. van Hulst
Journal of Luminescence
vol 128 issue 5-6 p1050–1052 jan 2 2008
doi:10.1016/j.jlumin.2007.11.027

Nanoscale Organization of the Pathogen Receptor DC-SIGN Mapped by Single-Molecule High-Resolution Fluorescence Microscopy

(abstract) (full pdf)
Bärbel I. de Bakker, Frank de Lange, Alessandra Cambi, Jeroen P. Korterik, Erik M. H. P. van Dijk, Niek F. van Hulst, Carl G. Figdor, and Maria F. Garcia-Parajo
ChemPhysChem
vol 8 issue 10 p1473-1480 July 16, 2007

Power-Law Blinking in the Fluorescence of Single Organic Molecules

(abstract) (full pdf)
Jacob P. Hoogenboom, Jordi Hernando, Erik M. H. P. van Dijk, Niek F. van Hulst, and Maria F. García-Parajó
ChemPhysChem
vol 8 issue 6, p823 - 833 march 23 2007

DNA-Based Molecular Wires: Multiple Emission Pathways of Individual Constructs

(abstract) (full pdf)
Gabriel Sánchez-Mosteiro, Erik M. H. P. van Dijk, Jordi Hernando, Mike Heilemann, Philip Tinnefeld, Markus Sauer, Felix Koberlin, Matthias Patting, Michael Wahl, Rainer Erdmann, Niek F. van Hulst, and Mariá F. García-Parajó
J. Phys. Chem. B
vol. 101 10.1021 p26349-26353 dec 05 2006

Effect of Disorder on Ultrafast Exciton Dynamics Probed by Single Molecule Spectroscopy

(abstract) (full pdf)
Jordi Hernando, Erik M. H. P. van Dijk, Jacob P. Hoogenboom, Juan-José García-López, David N. Reinhoudt, Mercedes Crego-Calama, María F. García-Parajó, and Niek F. van Hulst
Physical Review Letters
vol. 97 issue 21 p216403 nov 20 2006

Synthesis and Characterization of Long Perylenediimide Polymer Fibers: From Bulk to the Single-Molecule Level

(abstract) (full pdf)
Pieter A.J. De Witte, Jordi Hernando, Edda E. Neuteboom, Erik M.H.P. van Dijk, Stefan C.J. Meskers, Rene A.J. Janssen, Niek F. van Hulst, Roeland J.M. Nolte, María F. García-Parajó and Alan E. Rowan
J. Phys. Chem. B
Vol 110 No 15 p7803 - p7812 march 25, 2006

Power-Law-Distributed Dark States are the Main Pathway for Photobleaching of Single Organic Molecules

(abstract) (full pdf)
Jacob P. Hoogenboom, Erik M. H. P. van Dijk, Jordi Hernando, Niek F. van Hulst, and María F. García-Parajó
Physical Review Letters
vol. 95 097401 aug 26 2005

Near-Field Fluorescence Microscopy An Optical Nanotool to Study Protein Organization at the Cell Membrane

(abstract) (full pdf)
María F. García-Parajó, Bärbel I. de Bakker, Marjolein Koopman, Alessandra Cambi, Frank de Lange, Carl G. Figdor, and Niek F. van Hulst
NanoBiotechnology
Volume 1 Number 1 ISSN: 1551-1286 (Print) ISSN: 1551-1294 (Online) july 2005

Single-molecule pump-probe experiments reveal variations in ultrafast energy redistribution

(abstract) (full pdf)
E.M.H.P. van Dijk, J. Hernando, M.F. García-Parajó, and N.F. van Hulst
the journal of Chemical Physics
vol. 123 issue 6 p064703 may 2 2005

Energy Transfer in Single-Molecule Photonic Wires

(abstract) (external link to pdf)
María F. García-Parajó, Dr., Jordi Hernando, Dr., Gabriel Sanchez Mosteiro, Jacob P. Hoogenboom, Dr., Erik M. H. P. van Dijk, Dr., Niek. F. van Hulst, Prof.
ChemPhysChem
Volume 6, Issue 5, P819 - 827 apr 22 2005

Single-molecule pump-probe detection resolves ultrafast pathways in individual and coupled quantum systems

(abstract) (full pdf)
Erik M.H.P. van Dijk, Jordi Hernando, Juan-José García-López, Mercedes Crego-Calama, David N. Reinhoudt, Laurens Kuipers, María F. García-Parajó Niek F. van Hulst
Physical Review Letters
vol 94 p078302-1 - p078302-4 25 feb 2005

Molecular Printboards on Silicon Oxide: Lithographic Patterning of Cyclodextrin Monolayers with Multivalent, Fluorescent Guest Molecules

(abstract) (external link to pdf)
Alart Mulder, Steffen Onclin, Mária Péter, Jacob P. Hoogenboom, Hans Beijleveld, Jurjen ter Maat, María F. García-Parajó, Bart Jan Ravoo, Jurriaan Huskens, Niek F. van Hulst and David N. Reinhoudt
Small
vol 1 no 2 p242-p253 2005

Monolayer-functionalized microfluidics devices for optical sensing of acidity

(abstract) (full pdf)
P. Mela, S. Onclin, M. H. Goedbloed, S. Levi, M. F. García-Parajó, N. F. van Hulst, B. J. Ravoo, D. N. Reinhoudt and A. van den Berg
Lab. on a Chip
vol 5 no 2 p163 - p170 2005

Single Molecule Photobleaching Probes the Exciton Wave Function in a Multichromophoric System

(abstract) (full pdf)
J. Hernando, J.P. Hoogenboom, E.M.H.P. van Dijk, J.J. García-Lopez, M. Crego-Calama, D.N. Reinhoudt, N.F. van Hulst, and M.F. García-Parajó
Physical Review Letters
vol 93 no 23 p 236404 1-4 dec 3, 2004
doi:10.1103/PhysRevLett.93.236404

Photon Antibunching Proves Emission from a Single Subunit in the Autofluorescent Protein DsRed

(external link to pdf)
Gabriel Sánchez-Mosteiro, Majolein Koopman, Erik M. H. P. van Dijk, Jordi Hernando, Niek F. van Hulst, and María F. García-Parajó
ChemPhysChem
vol 5 issue 11 p1782 - p1785 nov 5 2004

Investigation of Perylene Photonic Wires by Combined Single-Molecule Fluorescence and Atomic Force Microscopy

(abstract) (external link to pdf)
Jordi Hernando, Pieter A. J. de Witte, Erik M. H.P van Dijk, Jeroen Korterik, Roeland J. M. Nolte, Alan E. Rowan, Maria F. García-Parajó, and Niek F. van Hulst
Angewandte Chemie
vol 43 issue 31 p4045 - p4049, aug 6 2004

Near-field scanning optical microscopy in liquid for high resolution single molecule detection on dendritic cells

(abstract) (full pdf)
M. Koopman, A. Cambi, B.I. de Bakker, B. Joosten, C.G. Figdor, N.F. van Hulst, M.F. García-Parajó
FEBS letters (Federation of European Biochemical Societies)
vol 573 (2004) issues 1-3 p6 - p10 july 6 2004

A Simple Approach to Sensor Discovery and Fabrication on Self-Assembled Monolayers on Glass

(abstract) (full pdf)
Lourdes Basabe-Desmonts, Joris Beld, Rebecca S. Zimmerman, Jordi Hernando, Petra Mela, María F. García Parajó, Niek F. van Hulst, Albert van den Berg, David N. Reinhoudt, and Mercedes Crego-Calama
Journal of the American Chemical Society
vol 126 no 23: p7293-p7299 jun 16 2004

Multistep Energy Transfer in Single Molecular Photonic Wires

(abstract) (full pdf)
Mike Heilemann, Philip Tinnefeld, Gabriël Sanchez Mosteiro, Maria García Parajó, Niek F. Van Hulst and Markus Sauer
Journal of the American Chemical Society
vol 126 no 21 p6514-6515 may 6 2004

Cell plasma membrane organization at nanometer scale studied with single molecule sensitive fluorescence microscopy


Koopman M, Cambi A, Joosten B, Garcia-Parajo MF, Figdor CG, van Hulst NF
Biophysical Journal
vol 86 (1): 363A-364A Part 2 Suppl. S, Jan 2004

Single molecule near-field fluorescence microsocopy reveals hierarchical organization of C-type lectins on dendritic cells


Garcia-Parajo MF, de Bakker B, Cambi A, de Lange F, Figdor C, van Hulst N
Biophysical Journal
vol 86 (1): 149A-149A Part 2 Suppl. S, Jan 2004

Probing polymers with single fluorescent molecules

(abstract) (full pdf)
Nikodem Tomczak, Renaud A.L. Vallée, Erik M.H.P. van Dijk, Maria García-Parajó, Laurens Kuipers, Niek F. van Hulst, G. Julius Vancso
European Polymer Journal
vol 40 p1001-1011 20 jan 2004

Shear force imaging of soft samples in liquid using a diving bell concept

(abstract) (full pdf)
M. Koopman, B. I. de Bakker, M. F. Garcia-Parajo, and N. F. van Hulst
Applied Physics Letters
Vol 83, Issue 24, pp. 5083-5085, Dec 15, 2003

Excitonic behavior of rhodamine dimers: A single-molecule study

(abstract) (full pdf)
Hernando J, van der Schaaf M, van Dijk EMHP, Sauer M, Garcia-Parajo MF, van Hulst NF
JOURNAL OF PHYSICAL CHEMISTRY A
vol 107 issue 1: p43-p52 JAN 9 2003

Film thickness effects on single molecule optical probing of polymers


Tomczak N, Vallee R, Garcia-Parajo MF, Kuipers L, van Hulst NF, Vancso JG
Polym. Mat.:Sci.Eng.
vol 88 p449-p450 (2003)

Single molecule studies of the red autofluorescent protein DsRed

(full pdf)
Garcia-Parajo MF, Koopman M, van Dijk EMHP, Subramaniam V, van Hulst NF
BIOPHYSICAL JOURNAL
vol 82 issue 1: p234 part 2 JAN 2002

The nature of fluorescence emission in the red fluorescent protein DsRed, revealed by single-molecule detection

(abstract) (full pdf)
Garcia-Parajo MF, Koopman M, van Dijk EMHP, Subramaniam V, van Hulst NF
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA (PNAS)
vol 98 issue 25: p14392-p14397 DEC 4 2001

Cell biology beyond the diffraction limit: near-field scanning optical microscopy

(abstract) (full pdf)
de Lange F, Cambi A, Huijbens R, de Bakker B, Rensen W, Garcia-Parajo M, van Hulst N, Figdor CG
JOURNAL OF CELL SCIENCE
vol 114 issue 23: p4153-p4160 DEC 2001

On the role of electromagnetic boundary conditions in single molecule fluorescence lifetime studies of dyes embedded in thin films

(abstract) (full pdf)
Vallee R, Tomczak N, Gersen H, van Dijk EMHP, Garcia-Parajo MF, Vancso GJ, van Hulst NF
CHEMICAL PHYSICS LETTERS
vol 348 issue 3-4: p161-p167 NOV 9 2001

Optical probing of single fluorescent molecules and proteins

(abstract) (external link to pdf)
Garcia-Parajo MF, Veerman JA, Bouwhuis R, Vallee R, van Hulst NF
CHEMPHYSCHEM
vol 2 issue 6: p347-p360 JUN 18 2001

Near-field effects in single molecule emission

(abstract)
Gersen H, Garcia-Parajo MF, Novotny L, Veerman JA, Kuipers L, Van Hulst NF
JOURNAL OF MICROSCOPY-OXFORD
vol 202: p374-p378 part 2 MAY 2001

Single molecules in the near field.


van Hulst NF, Garcia-Parajo MF, Veerman JA, Gersen H
ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY
vol 221: p169-ANYL part 1 APR 1 2001

Single-molecule probing of nanoscale polymer dynamics.


Garcia-Parajo MF, Veerman JA, Bouwhuis R, Vallee R, Vancso JG, van Hulst NF
ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY
vol 221: p175-PHYS part 2 APR 1 2001

Single molecule studies of living colors and transmembrane proteins on cells.


Garcia-Parajo MF, de Bakker BI, Koopman M, Figdor C, van Hulst NF
ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY
vol 221: p379-PHYS part 2 APR 1 2001

Moulded photoplastic probes for near-field optical applications

(abstract)
Kim BJ, Flamma JW, Ten Have ES, Garcia-Parajo MF, Van Hulst NF, Brugger J
JOURNAL OF MICROSCOPY-OXFORD
vol 202: p16-p21 part 1 APR 2001

Looking at the photodynamics of individual fluorescent molecules and proteins

(abstract)
Garcia-Parajo MF, Veerman JA, Kuipers L, van Hulst NF
PURE AND APPLIED CHEMISTRY
vol 73 issue 3: p431-p434 MAR 2001

Single molecule studies of living colours.

(full pdf)
Garcia-Parajo MF, Koopman M, de Bakker BI, van Hulst NF
BIOPHYSICAL JOURNAL
vol 80 issue 1: p655.12 part 2 JAN 2001

Study of individual transmembrane proteins using a combined confocal/near field optical microscope.

(full pdf)
de Bakker BI, Garcia-Parajo MF, Rensen WHJ, van Hulst NF, de Lange F, Cambi A, Figdor CG
BIOPHYSICAL JOURNAL
vol 80 issue 1: p655.13 part 2 JAN 2001

Single molecule probing of nanoscale polymer dynamics


R.A.L. Vallee, N. Tomczak, M.F. Garcia-Parajo, G.J. Vancso, N.F. van Hulst
Polymer Preprints
2001

Influencing the angular emission of a single molecule

(abstract) (full pdf)
Gersen H, Garcia-Parajo MF, Novotny L, Veerman JA, Kuipers L, van Hulst NF
PHYSICAL REVIEW LETTERS
vol 85 issue 25: p5312-p5315 DEC 18 2000

Real-time light-driven dynamics of the fluorescence emission in single green fluorescent protein molecules

(abstract)
Garcia-Parajo MF, Segers-Nolten GMJ, Veerman JA, Greve J, van Hulst NF
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA (PNAS)
vol 97 issue 13: p7237-p7242 JUN 20 2000

Analysis of individual (macro)molecules and proteins using near-field optics

(abstract) (full pdf)
van Hulst NF, Veerman JA, Garcia-Parajo MF, Kuipers L
JOURNAL OF CHEMICAL PHYSICS
vol 112 issue 18: p7799-p7810 MAY 8 2000

Real time light-driven dynamics of the fluorescence emission in individual green fluorescent proteins


Garcia-Parajo MF, Segers-Nolten GMJ, Veerman JA, van Hulst NF
BIOPHYSICAL JOURNAL
vol 78 issue 1: p2264Pos part 2 JAN 2000

Time-varying triplet state lifetimes of single molecules

(abstract) (full pdf)
Veerman JA, Garcia-Parajo MF, Kuipers L, van Hulst NF
PHYSICAL REVIEW LETTERS
vol 83 issue 11: p2155-p2158 SEP 13 1999

Visualising individual green fluorescent proteins with a near field optical microscope

(abstract)
Garcia-Parajo MF, Veerman JA, Segers-Nolten GMJ, de Grooth BG, Greve J, van Hulst NF
CYTOMETRY
vol 36 issue 3: p239-p246 JUL 1 1999

Single molecule mapping of the optical field distribution of probes for near-field microscopy

(abstract) (full pdf)
Veerman JA, Garcia-Parajo MF, Kuipers L, Van Hulst NF
JOURNAL OF MICROSCOPY-OXFORD
vol 194: p477-p482 part 2-3 MAY-JUN 1999

Near-field optical microscopy for DNA studies at the single molecular level

(abstract) (full pdf)
Garcia-Parajo MF, Veerman JA, van Noort SJT, de Grooth BG, Greve J, van Hulst NF
BIOIMAGING
vol 6 issue 1: p43-p53 MAR 1998

Tuning fork shear-force feedback

(abstract) (full pdf)
Ruiter AGT, van der Werf KO, Veerman JA, Garcia-Parajo MF, Rensen WHJ, van Hulst NF
ULTRAMICROSCOPY
vol 71 issue 1-4: p149-p157 MAR 1998

Near-field optical and shear-force microscopy of single fluorophores and DNA molecules

(abstract) (full pdf)
Garcia-Parajo MF, Veerman JA, Ruiter AGT, van Hulst NF
ULTRAMICROSCOPY
vol 71 issue 1-4: p311-p319 MAR 1998

Single molecule rotational and translational diffusion observed by near-field scanning optical microscopy

(abstract)
Ruiter AGT, Veerman JA, GarciaParajo MF, vanHulst NF
JOURNAL OF PHYSICAL CHEMISTRY A
vol 101 issue 40: p7318-p7323 OCT 2 1997

Near-field fluorescence imaging of genetic material: Toward the molecular limit

(abstract)
vanHulst NF, GarciaParajo MF, Moers MHP, Veerman JA, Ruiter AGT
JOURNAL OF STRUCTURAL BIOLOGY
vol 119 issue 2: p222-p231 1997


Books by María García-Parajó


Ultrafast spectroscopy of single molecules

(pdf)
Erik van Dijk, Mark te Paske, Jordi Hernando, Jacob Hoogenboom, Maria Garcia-Parajo, Niek van Hulst

Springer Series in Chemical Physics:
Ultrafast Phenomena XV:
Proc. of the 15th International Conference, Pacific Grove/CA, USA, July 30 - August 4, 2006

vol.88, p.231-233, 2007
Printable version