Optical Sciences

Biomolecules and nanostructures

The Optical Sciences group studies the interaction of light and matter. Our current focus is on detection and sensing/imaging with an emphasis on the development of integrated photonics. We are part of Twente University's Department of Science and Technology and member of the MESA+ institute.


Adhesisomes; dynamic multimolecular structures that mediate cell communication and adhesion in the immune system

  • Marjolein Koopman - Former member

  • The aim of this project is to unravel the dynamics of adhesisomes at cell surfaces employing SNOM based technology. We expect to get a clear insight in the organisation and dynamics of these structures; diffusion and orientation of individual and clustered components, adhesisome assembly, recruitment of signalling molecules, adhesisome half life, and disassembly. Fluorescent labelling of one or more components of the adhesisome employing expression vectors containing GFP cDNA's is essential to our approach. Using SNOM we will be able to combine high resolution wit low background, essential to reduce the cytoplasmic autofluorescence. Although this approach could be used to study any ligand/receptor system important in the immune system, we here want to focus on the integrin LFA-1 and its ligands ICAM-1, -2 and -3, because we have a long-standing interest in these molecules and a large number of tools are available.


    The following articles have been published regarding this project:

    Near-Field Fluorescence Microscopy An Optical Nanotool to Study Protein Organization at the Cell Membrane

    (abstract) (full pdf)
    María F. García-Parajó, Bärbel I. de Bakker, Marjolein Koopman, Alessandra Cambi, Frank de Lange, Carl G. Figdor, and Niek F. van Hulst
    Volume 1 Number 1 ISSN: 1551-1286 (Print) ISSN: 1551-1294 (Online) july 2005

    How C-type lectins detect pathogens

    (abstract) (full pdf)
    Cambi A, Koopman M, Figdor CG
    Cellular Microbiology
    vol. 7 issue 4 p 481-488 april 2005

    Near-field scanning optical microscopy in liquid for high resolution single molecule detection on dendritic cells

    (abstract) (full pdf)
    M. Koopman, A. Cambi, B.I. de Bakker, B. Joosten, C.G. Figdor, N.F. van Hulst, M.F. García-Parajó
    FEBS letters (Federation of European Biochemical Societies)
    vol 573 (2004) issues 1-3 p6 - p10 july 6 2004

    Shear force imaging of soft samples in liquid using a diving bell concept

    (abstract) (full pdf)
    M. Koopman, B. I. de Bakker, M. F. Garcia-Parajo, and N. F. van Hulst
    Applied Physics Letters
    Vol 83, Issue 24, pp. 5083-5085, Dec 15, 2003

    Single molecule studies of living colors and transmembrane proteins on cells.

    Garcia-Parajo MF, de Bakker BI, Koopman M, Figdor C, van Hulst NF
    vol 221: p379-PHYS part 2 APR 1 2001
    Printable version