Optical Sciences

Biomolecules and nanostructures

The Optical Sciences group studies the interaction of light and matter. Our current focus is on detection and sensing/imaging with an emphasis on the development of integrated photonics. We are part of Twente University's Department of Science and Technology and member of the MESA+ institute.

 

Near field optical microscopy and spectroscopy




The aperture type Near-field Scanning Optical Microscope (NSOM), realised in 1993, has been improved and applied in the study of several physical, biophysical and chemical structures:

  • Polarised fluorescence of Langmuir-Blodgett mono-layers showing the molecular orientation of the polymer backbone and degree of polymerisation on nm-scale.

  • Detection of fluorescent DNA probes on human metaphase chromosomes with a resolution superior to conventional epi-fluorescence microscopy. A resolution of better than 80 nm has been realised on metaphase chromosomes while a topographic image (50 nm resolution) is obtained simultaneously with the optical image. To our knowledge this is the first useful biological application of NSOM.

  • First detection of Raman spectra with a near-field probe on diamond and polystyrene films by long time integration using a photon counting CCD camera.

  • Fluorescence of single molecules with the near-field scanning optical microscope has been observed and is the main item of current investigations.


  • This FOM-BR project has been concluded with a thesis in 1995 and is continued by a new project focused on "Photodynamics of Single Molecules", financed by FOM-AQ following the 1994 evaluation.


    Articles

    The following articles have been published regarding this project:

    Fluorescence in situ hybridization on human metaphase chromosomes detected by near-field scanning optical microscopy

    (abstract)
    Moers MHP, Kalle WHJ, Ruiter AGT, Wiegant JCAG, Raap AK, Greve J, DeGrooth BG, VanHulst NF
    JOURNAL OF MICROSCOPY-OXFORD
    vol 182: p40-p45 part 1 APR 1996

    Biological application of near-field optical microscopy


    vanHulst NF, Moers MHP
    IEEE ENGINEERING IN MEDICINE AND BIOLOGY MAGAZINE
    vol 15 issue 1: p51-p58 JAN-FEB 1996

    Detection of fluorescence in situ hybridization on human metaphase chromosomes by near-field scanning optical microscopy

    (abstract)
    Moers MHP, Ruiter AGT, Jalocha A, vanHulst NF
    ULTRAMICROSCOPY
    vol 61 issue 1-4: p279-p283 DEC 1995

    FLUORESCENCE IN-SITU HYBRIDIZATION ON HUMAN METAPHASE CHROMOSOMES DETECTED BY NEAR-FIELD MICROSCOPY


    MOERS MHP, RUITER AGT, VANHULST NF
    ZOOLOGICAL STUDIES
    vol 34: p21-p22 APR 1995

    OPTICAL CONTRAST IN NEAR-FIELD TECHNIQUES

    (abstract) (full pdf)
    MOERS MHP, VANHULST NF, RUITER AGT, BOLGER B
    ULTRAMICROSCOPY
    vol 57 issue 2-3: p298-p302 FEB 1995

    POLY(DIACETYLENE) MONOLAYERS STUDIED WITH A FLUORESCENCE SCANNING NEAR-FIELD OPTICAL MICROSCOPE

    (abstract)
    MOERS MHP, GAUB HE, VANHULST NF
    LANGMUIR
    vol 10 issue 8: p2774-p2777 AUG 1994

    PHOTON SCANNING TUNNELING MICROSCOPE IN COMBINATION WITH A FORCE MICROSCOPE

    (abstract) (full pdf)
    MOERS MHP, TACK RG, VANHULST NF, BOLGER B
    JOURNAL OF APPLIED PHYSICS
    vol 75 issue 3: p1254-p1257 FEB 1 1994

    A COMBINED NEAR-FIELD OPTICAL AND FORCE MICROSCOPE

    (abstract)
    MOERS MHP, TACK RG, VANHULST NF, BOLGER B
    SCANNING MICROSCOPY
    vol 7 issue 3: p789-p792 SEP 1993

    NEAR-FIELD OPTICAL MICROSCOPY IN TRANSMISSION AND REFLECTION MODES IN COMBINATION WITH FORCE MICROSCOPY

    (abstract)
    VANHULST NF, MOERS MHP, BOLGER B
    JOURNAL OF MICROSCOPY-OXFORD
    vol 171: p95-p105 part 2 AUG 1993

    COMBINED SCANNING NEAR-FIELD OPTICAL AND FORCE MICROSCOPY


    VANHULST NF, MOERS MHP, TACK RG, BOLGER B
    MICROSCOPY RESEARCH AND TECHNIQUE
    vol 25 issue 2: p177-p178 JUN 1 1993

    NEAR-FIELD OPTICAL MICROSCOPE USING A SILICON-NITRIDE PROBE

    (abstract) (full pdf)
    VANHULST NF, MOERS MHP, NOORDMAN OFJ, TACK RG, SEGERINK FB, BOLGER B
    APPLIED PHYSICS LETTERS
    vol 62 issue 5: p461-p463 FEB 1 1993
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